Imaging and Histology Core
The University of Texas Dallas’ newly established Imaging and Histology Core facility is located in the Natural Science and Engineering Research Laboratory (NSERL) with 3090 square feet of renovated and dedicated lab space. The core houses state-of-the-art instruments and technology for researchers on-campus and external users to provide affordable support for basic research and translational research in biological and interdisciplinary sciences. This core facility is operated by Office of Research and employs dedicated full-time technical staff to operate and maintain the equipment. The Histology Core provides support for processing and embedding of tissue samples, sectioning and slide preparation, cell culture, and sample imaging. The Imaging Core houses advanced imaging systems, including an electron microscope, confocal laser scanning microscopes, a multiphoton intravital imaging system, and various other optical microscopes.
Histology and Cell Culture Equipment
- Leica ASP300 S: Fully enclosed Tissue Processor
- Leica EM UC7: Ultramicrotome used for sample preparation for electron microscopy
- Leica RM2235: Manual microtome
- Leica CM1860: Clinical Cryostat
- Leica EM FC7: Cryochamber used for sample preparation for electron microscopy
- HistoCore ARCADIA C and H: Cold and hot paraffin embedding stations
- Leica EM ACE200: Carbon coater used for sample preparation for electron microscopy
- Leica EM KMR3 Glass Knifemaker used for sample preparation for electron microscopy
- NuAire Class II, Type A2 Biological Safety Cabinet
- NuAire Direct Heat In-Vitrocell Air-Jacketed CO2 Incubators
Olympus and Zeiss Imaging Instruments
The Olympus imaging equipment is part of the University of Texas Dallas / Olympus Imaging Reference Center. This Center is a collaborative effort between the University of Texas Dallas and Olympus establishing a state-of-the-art imaging core, including top-end Olympus imaging facilities, hands on training from Olympus staff, an Imaging Summer School run cooperatively by Olympus and UTD, and regular beta testing of new Olympus imaging equipment.
- Olympus MPE-RS TWIN: Intravital Multiphoton Fluorescence Microscope with dual multiphoton laser system for simultaneous scanning and excitation
- Olympus FV3000RS: Laser Scanning Confocal Microscope equipped with live cell imaging capacity and a fully automated stage for whole tissue stitching. The system also includes objectives for cleared tissue imaging compatible with SCA\E and Clarity techniques.
- Olympus SD-OSR: Spinning Disk Confocal Microscope equipped with live cell imaging capacity
- Olympus IX83 TIRF: Total Internal Reflectance Fluorescence system
- Olympus VS120: 100-Slide Scanning System
- Imaris software work-station for visualizing and analyzing imaged data
- Zeiss E-SEM: Environmental Scanning Electron Microscope
Service and Rates
- All fees in $/hr unless otherwise noted.
- User Fee – User fee for using an instrument with some assistance from staff.
- Service Fee – Staff runs the sample on the various instruments and provides the results.
- Training Fee – $50/hr per trainee per instrument.
|Imaging Instruments||Internal User Fee||Internal Service Fee|
|VS120 Slide Scanner||$15||$37|
|Histology Instrument||Internal User Fee|
|Tissue processor & Embedder||$9|
To reserve an instrument please visit the Lab Resources Scheduler. Name of the Faculty Supervisor/Sponsor and a valid Cost Center number is required to complete the reservation.
- Users must make reservations before using the instruments.
- Reservations must be cancelled at least 24 hours before the starting time of the reservation. Otherwise, the users will be charged for the reserved time slot.
- Users are responsible for charges of unused reservation hours because of being late or for no-shows.
- Users must not be more than 30 minutes late to prevent other users using your reserved time slot at your expense.
- If a user cannot use the reserved slot for emergency reasons please bring it to the staff’s attention.